Rapid communication: a TaqI restriction fragment length polymorphism in the ovine collagenase gene.

Polymorphism. Tag1 restriction fragment length polymorphism (RFLP) in the collagenase (CLG) of sheep. Source and Description of Probe. A 2.05-kb cDNA insert was excised from a pSP64 clone using HindIII and SmaI. The insert contains the complete coding sequence of the human fibroblast collagenase gene, as described by Angel et al. (1987). Method of Detection. Prehybridization and hybridization solutions contained 6 x SET buffer, 1% SDS, 5 x Denhardt’s, 10% dextran sulfate, and 100 pgimL of salmon sperm DNA. Filters were prehybridized for approximately 5 h and hybridized for 20 h at 55°C using 1.0 x lo6 cpm of nick-translated probeimL of hybridization buffer. The filters were washed in 2 x SSC/.5% SDS two times for 20 min at room temperature and .1 x SSC/.5% SDS four times for 30 min at 55°C. Description of Polymorphism. Four codominantly inherited bands were detected using TuqI and the sizes were 9.8, 5.2, 4.2, and 3.6 kb. Four monomorphic